Link reaction and Krebs Cycle

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  • Link reaction:
    • Takes place within the fluid MATRIX of the MITOCHONDRION.
    • Converts pyruvic acid to ACETYL COA. Coenzyme A is RECYCLED.
    • Produces 2 HYDROGEN and NADH2. Produces CARBON DIOXIDE.
    • Krebs Cycle:
      • 1. 4C OXALOACETIC ACID                    -->         6C CITRIC ACID
        • 2C acetyl group of acetyl CoA combines with 4C oxaloacetic acid to form the 6C citric acid.
      • 2. 6C CITRIC ACID --> 5C KETO GLUTARIC ACID
        • 6C Citric acid is OXIDISED to 5C sugar. DECARBOXYLATION + DEHYDROGENATION. Produces CO2, HYDROGEN and NADH2.
      • 3. 5C KETO GLUTARIC ACID --> 4C SUCCINIC ACID
        • 5C OXIDISED to 4C. Generates NADH2 and CO2. DECARBOXYLATION, DEHYDROGENATION. 2 HYDROGENS produced, ATP produced by SUBSTRATE-LEVEL PHOSPHORYLATION.
      • 4. 4C SUCCINIC ACID --> 4C MALIC ACID
        • OXIDISED. Hydrogen carrier = FAD. 2 HYDROGENS produced and FADH2.
      • 5. 4C MALIC ACID --> 4C OXALOACETIC ACID
        • OXIDISED back to the 4C ocaloacetic acid, enabling the cycle to start again. 2 HYDROGENS produced, NADH2 produced.

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