insulin and golden rice
- Created by: vezting
- Created on: 24-02-16 08:08
View mindmap
- insulin and golden rice
- insulin
- 1: gene from human insulin identified and isolated from pancreatic cells using restriction enzymes or reverse transcriptidase
- reverse transcriptidase makes cDNA which is then cut by rest. enzymes leaving sticky ends
- 2: a plasmid is cut with same rest. enzymes
- 3: insulin gene inserted into tetracilin resistant gene in the plasmid
- 4: plasmids placed on amplicillin agar and all should grow
- 5: then they are placed on tetracillin agar, only non-resistant will grow and we weant the non-growth colony
- + identical so less risk of rejection +cheaper and faster to produce + avoids religious and ethical conflict
- 1: gene from human insulin identified and isolated from pancreatic cells using restriction enzymes or reverse transcriptidase
- golden rice
- 1:PSY gene from maize and CRTL gene from soil bacterium are isolated using restriction enzymes
- 2: plasmid removed from agrobacterium tumerfaciens bacteria and cut using same rest. enzyme
- 3: PSY and CRTL genes inserted into plasmid
- 4: recombinant plasmid inserted back into bacteria
- 5: rice pant cells incubated with bacteria which infects plants abd transfroms DNA of rice plant
- rice plants grown on selective medium so only transformed plants grow as a marker gene is needed
- + plant now produces beta-carotene which reduces vit. A defficiencies
- insulin
Comments
No comments have yet been made