Immobilised enzymes

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  • Immobilised enzymes
    • Enzyme molecules that are fixed to an inert Matrix/alginate bead 
    • They are more stable at higher temperatures 
      • therefore reaction rates may be faster by usinghigher temps
    • They can tolerate wider range of pH 
    • They are more easily recovered for re-use/separated from product 
    • Several enzymes with different pH or temp optimum can be used at one time.
    • Reaction can be more easily controlled by adding or removing enzymes 
    • They are specific so can select one type of molecule in a mixture 
      • So can be used for rapid detection of biologically important molecules 
    • They can also accurately measure the quantities present / are sensitive 
    • Used in medical diagnosis 
      • e.g. diabetes
      • And environmental monitoring 
    •  some use a transducer to generate an electrical impulse that can be measured with a meter. eg. Blood sugar meter as used by diabetics 


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