Immobilised enzymes
- Created by: katie
- Created on: 24-03-13 10:16
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- Immobilised enzymes
- Enzyme molecules that are fixed to an inert Matrix/alginate bead
-
They are more stable at higher temperatures
- therefore reaction rates may be faster by usinghigher temps
- They can tolerate wider range of pH
- They are more easily recovered for re-use/separated from product
- Several enzymes with different pH or temp optimum can be used at one time.
- Reaction can be more easily controlled by adding or removing enzymes
-
They are specific so can select one type of molecule
in a mixture
- So can be used for rapid detection of biologically important molecules
- They can also accurately measure the quantities present / are sensitive
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Used in medical diagnosis
- e.g. diabetes
- And environmental monitoring
- some use a transducer to generate an electrical impulse that can be measured with a meter. eg. Blood sugar meter as used by diabetics
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