RNA Polymerase II Promoter

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  • Created by: Ellie0o0o
  • Created on: 28-04-15 09:04
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  • Eukaryotic Pol II Promoters
    • Core Promoter
      • Proximal to, sometimes overlapping the start of the transcription site
      • Cis-acting
      • Sufficient for basal levels of transcription in vivo
      • Start site
        • Always +1
        • Usually flanked by Cs and Ts
        • TATA Box
          • Equivalent to the -10 sequence in bacteria
          • ~ 25-30 bps upstream
          • AT rich
          • Flanked by Cs and Gs
          • Initial target site for assembly of polymerase for basal transcription
          • Initiators are sometimes also present
            • Can have initiators or TATA box, both or neither
        • Initiators are sometimes also present
          • Can have initiators or TATA box, both or neither
    • Regulatory Region
      • Usually distal
      • Can be in-trans
      • Alters transcription from basal level
    • Modular

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