Factors affecting enzymes
- Created by: jupiter1997
- Created on: 11-05-15 13:19
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- Factors affecting enzymes
- Temperature
- Kinetic energy increases
- More successful collisions
- Optimum pH (40 degrees)
- Enzyme denatures -bonds break and tertiary form unravels
- Optimum pH (40 degrees)
- More successful collisions
- Kinetic energy increases
- pH
- Enzymes have an optimum pH e.g. pepsin (pH2), amylase (pH7)
- Small changes lead to inactivation. Extreme changes leads to denaturation
- If active site has too many H+ ions, the active site and substrate have same charge and repel
- Small changes lead to inactivation. Extreme changes leads to denaturation
- Enzymes have an optimum pH e.g. pepsin (pH2), amylase (pH7)
- Substrate Concentration
- As the substrate concentration increases, the active sites will all become full, and rate is at its maximum
- Enzyme Concentration
- Increasing enzyme concentration, increases rate
- Turnover number
- Increasing enzyme concentration, increases rate
- Competitive Inhibitors
- e.g. malonic acid
- Competes with the substrate for the active site preventing ESCs being formed
- If you inrease substrate concentration, it'll reduce the effect of the inhibitor
- Non-competitive inhibitors
- e.g. cyanide
- Binds to allosteric site, causing the active site to change shape, meaning the site is no longer complementary to the substrate
- Temperature
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