Enzyme concentration core practical

  • Created by: Emling
  • Created on: 19-03-19 09:52
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  • Enzyme concentration- core practical
    • Variables
      • Independent
        • Concentration of yeast
      • Dependent
        • Volume of gas produced
      • Control
        • Temperature
          • Temperature can denature enzymes
        • Volume of solution
          • To make results comparable
        • pH of water
          • Enzymes have an optimum pH where they work best
        • Time measured for
          • Make results comparable
        • Volume of hydrogen peroxide
          • Substrate that breaks down the enzymes
    • Equiptment
      • Yeast
      • Scales
      • Distilled water
      • String rod
      • Syringe
      • Measuring cylinders
      • Delivery tube
      • Conical flask
      • Stop watch
      • Clamp stands
    • Method
      • 1. Measure out 4g of yeast
      • 2. Put yeast in 100ml of distilled water and stir
      • 3. Fill up a bowl with water
      • 4. Fill up the measuring cylinder with water and put into bowl
      • 5. Clamp it and a conical flask in place
      • 6. Syringe yeast solution into conical flask.
      • 7. Measure out 4cm cubed of hydrogen peroxide and put in conical flask replacing the bung
      • 8. Start the stopwatch
      • 10. Read the volume of water from the measuring cylinder for 120 seconds

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