Effect of Caffeine on Daphnia- Core Practicle
- Created by: Emling
- Created on: 29-10-18 11:44
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- Effect of Caffeine on Daphnia- Core Practicle
- Variables
- Independent
- Pond water or caffeine solution
- Dependent
- Heart rate of the Daphnia
- Control
- Volume of liquid
- Volume can contain more caffeine which can affect heart rate
- Daphina that we use
- Different Daphnia have different heart rates due to age etc
- Temperature of the Daphnia
- Daphnia are Poikilothermic and cant control their temperature- increased heat can cause stress and increase heart rate
- How long the microscope lamp is on for
- Daphnia are Poikilothermic
- Movement of the Daphnia
- More it moves- the more the heart rate increases
- Volume of liquid
- Independent
- Apparatus
- Daphnia
- Cavity slides
- Dropping pipets
- Caffeine solution
- Cotton wool
- Stop watch
- Paper towels
- Microscope
- Hand clicker
- Method
- 1. Set up microscope
- 2. Pick up Dapnia using dropping pipet
- 3. Put daphnia on cavity slide
- 4. Immobalsie with cotton wool
- 5.Adjust microscope focuse
- 6.Turn on lamp
- 7. Count Daphnia heart rate
- 8. Repeat experiment
- Ethics
- Daphina are living organisms so we need to be careful when conducting an experiment with them
- Variables
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