Effect of Caffeine on Daphnia- Core Practicle 

  • Created by: Emling
  • Created on: 29-10-18 11:44
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  • Effect of Caffeine on Daphnia- Core Practicle
    • Variables
      • Independent
        • Pond water or caffeine solution
      • Dependent
        • Heart rate of the Daphnia
      • Control
        • Volume of liquid
          • Volume can contain more caffeine which can affect heart rate
        • Daphina that we use
          • Different Daphnia have different heart rates due to age etc
        • Temperature of the Daphnia
          • Daphnia are Poikilothermic and cant control their temperature- increased heat can cause stress and increase heart rate
        • How long the microscope lamp is on for
          • Daphnia are Poikilothermic
        • Movement of the Daphnia
          • More it moves- the more the heart rate increases
    • Apparatus
      • Daphnia
      • Cavity slides
      • Dropping pipets
      • Caffeine solution
      • Cotton wool
      • Stop watch
      • Paper towels
      • Microscope
      • Hand clicker
    • Method
      • 1. Set up microscope
      • 2. Pick up Dapnia using dropping pipet
      • 3. Put daphnia on cavity slide
      • 4. Immobalsie with cotton wool
      • 5.Adjust microscope focuse
      • 6.Turn on lamp
      • 7. Count Daphnia heart rate
      • 8. Repeat experiment
    • Ethics
      • Daphina are living organisms so we need to be careful when conducting an experiment with them

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