DNA amplification

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  • DNA amplification
    • Polymerase Chain Reaction
      • Sample obtained
      • Sample treated with detergent
        • Cells break open and DNA is released
      • Sample added to test tube with DNA polymerase, primers w/ fluorescent markers and nucleotides
      • Heated at 95 degrees.
        • DNA separates into 2 strands
      • Heated at 55 degrees
        • Primers attach at start of STR
      • Heated at 70 degrees
        • DNA polymerase attaches, nucleotides are added, DNA and STR are replicated

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