Enzyme/substrate concentration

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  • Concentration effects
    • Increased substrate
      • Collisions more frequent
        • Thus more complexes form
          • Max rate: active sites all occupied
            • Further increase has no effect
    • Increased enzyme
      • More active sites; more complexes
        • All substrate molecules occupying
          • Max possible rate for fixed s concentration
    • Initial reaction rate
      • Highest when e and s mixed
        • Substrate molecules used up
          • Collisions decrease; r rate decreases
        • Max possible reaction rate
  • Max possible rate for fixed s concentration

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