commercial biotechnology
- Created by: vezting
- Created on: 19-10-15 11:02
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- commercial biotechnology
- fermenter
- pressure vents reduce gas build up
- air inlet provides sterile air and oxygen for respiration
- mixing blades make collisions more likely
- water jacket allows circulation of water around fermenter to regulate temp
- outlet tap for draining fermenter
- motor rotates the blades to mix the culture evenly
- inlet for the addition of nutrients
- electronic probes for measuring levels, pH, nutrients, oxygen
- air outlet bubbles water through mixture
- Conditions
- temperature: too hot denature, too cold growth too slow
- type and time of nutrients added manipulates primary or secondary metabolites
- lack of oxygen will lead to unwanted products from anaerobic respiration
- changes in pH can reduce enzyme activity and therefore growth rates
- cultures
- batch
- nutrient levels decline with time
- easy to set up and maintain
- if contamination occurs only one batch is lost
- for secondary metabolites
- continuous
- nutrients constantly added
- set up is harder
- contamination causes huge loss
- for primary metabolites
- batch
- asepsis
- asepsis is the absence of unwanted organisms
- unwanted microorganisms compete for space, reduce yield, cause spoilage, produce toxic chemicals,
- laboratory (small scale)
- apparatus sterilised, carried out in fume cupboard, cultures kept closed
- large scale
- washing and sterilising equipment and pipes
- stainless steel surfaces which is polished so things cant stick
- sterilising nutrient media
- fine filtering inlet pipes
- asepsis is the absence of unwanted organisms
- fermenter
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