Co-activators and chromatin structure
- Created by: natasha8sherry
- Created on: 10-11-13 16:10
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- Co-Activators and Chromatin remodelling
- ATP-dependent
- 4 families
- E.g. SWI/SNF - ubiquitous across eukaryotes - not always positive regulation
- SWI/SNF remodelling - shown in DNase I footprinting studies
- Add histones to DNA template - the position on the DNA will be the same in every experiment
- DNase added, cuts in same way due to protection of DNA by histones - gives 10bp ladder
- Addition of SWI/SNF + ATP --> disruption = further DNAse digestion
- Gel shift experiments indicate nucleosomes are still present
- SWI/SNF helps TBP to bind!
- SWI/SNF alters nucleosomal structure facilitating binding of TBP
- SWI/SNF remodelling - shown in DNase I footprinting studies
- Can disrupt chromatin structure in many ways
- Movement of nucleosomes relative to DNA template (sliding beads along string)
- Eviction of octameric nucleosomes from DNA
- NB. nucleosome = DNA wound around 8 histone protein cores
- Eviction of individual histones
- Exchange of histone proteins
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