Biochemical tests
- Created by: vezting
- Created on: 26-12-15 10:54
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- biochemical tests
- proteins
- few drops sodium hydroxide to make alkaline solution
- add copper (II) sulfate solution
- positive:Purple Negative: stay blue - colours can be pale
- reducing sugars
- reducing sugars include monosaccharides and disaccharides
- add benedicts reagent
- heat, do not boil
- negative- Blue positive - green, yellow, orange, brick red, (strength means more present)
- non- reducing sugars
- EG sucrose
- boil with hydrochloric acid
- neutralise with sodium hydrogen carbonate
- carry out reducing sugar steps
- starch
- add iodine to potassium iodide solution
- add to solution
- negative- orange/brown positive: dark blue/ black
- lipids
- add ethanol to sample
- shake for minute then add water
- negative- clear, positive: milky (the milkier it is the more of the sample present)
- proteins
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