AS LEVEL- Biology- Basic components of living systems (Microscopes 2)
- Created by: Ammarah123
- Created on: 01-11-18 17:15
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- Electron microscope
- Basic components of living systems (Microscopes 2)
- Lazer Scanning Confocal microscope
- Uses lazer beams to scan a specimen which is usually tagged with a fluorescent dye
- The lazer causes the dye to give off light. This light is then focused through a pinhole onto a detector. The detector is hooked up to a computer which generates an image
- The image produced can be 3D
- They can be used to look at objects at different depths in thick specimens
- The pinhole means that any out-of- focus light is blocked, so the image produced is more clearer than a normal light microscope
- Lazer Scanning Confocal microscope
- Preparation
- Fixing- Using chemicals to preserve to prevent decomposition
- Dehydration- Removes water + prevents vapourisation in the vaccum which would damage the specimen
- Staining with heavy metals- Metals are used to create contrast in the electron beams
- 2 types; TER + SER
- Transmission Electron Microscope (TEM)
- Smaller organelles can be seen eg. ribosomes , RER
- Cost = Expensive
- Size = Big
- Sample preparation = Complex
- Dead or Alive? Dead
- Vacuum? Yes
- Colour of specimen = Black + white
- Magnification = Over x500,000
- Resolution = 3-10nm
- Scanning Electron Microscope (SEM)
- Size = Big
- 3D image is produced + surface detail can be seen
- Resolution = 3-10nm
- Magnification = Over x500,000
- Colour of specimen = Black + white
- Vacuum? Yes
- Dead or Alive? Dead
- Sample preparation = Complex
- Cost = Expensive
- Transmission Electron Microscope (TEM)
- Basic components of living systems (Microscopes 2)
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