The enzyme beta-galactosidase which cleaves lactose into glucose and galactose.
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What does the gene lacY code for?
The enzyme permease which takes lactose into the cell.
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What does the lacA gene code for?
Encodes a transacetylase which seems to not be required for lactose metabolism.
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What are regulatory genes?
A class of genes that control the expression of other genes through the properties of their gene products.
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What do lacI- mtations lead to?
Production of a non-functional repressor protein that cannot bind to the operator site lacO.
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What are lacOc mutations?
Changes in the nucleotide sequence of the lacO site that prevent the normal lac repressor protein from binding.
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What do lacIs mutations lead to?
Production of a mutant repressor protein that can bind to the lacO site but is insensitive to the inducer lactose.
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Why is the lac promoter not stimulated at high glucose concentrations?
The high concentrations of ATP mean that the cell does not form cAMP-CAP complexes.
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What happens as the glucose concentration falls?
The concentration of cAMP goes up and it binds to CAP forming the cAMP-CAP complex which acts positively on the lac promoter to stimulate transcription.
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Other cards in this set
Card 2
Front
What does the gene lacY code for?
Back
The enzyme permease which takes lactose into the cell.
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