-Add Benedict's Reagent to the sample and heat for 5 minutes. If the Benedict's Reagent does not change colour (the solution reamins blue) then a reducing sugar is not present.
-Add a few drops of dilute hydrochloric acid in another test tube with another component of the sample in it and heat the solution. The dilute hydrochloric acid will hydrolyse any disaccharide present into it's monosaccharide components.
-Add some Sodium Hydrocarbonate Solution to the solution in order to neutralise the Hydrochloric Acid (Benedict's Reagent doesn't work in acidic conditions.
-Test the solution with pH paper to check that the solution is alkaline.
-Re-test the resulting solution by adding Benedict's Reagent and heating it.
-If a Non-Reducing Sugar was present in the original sample, the Benedict's reagent will now turn orange-brown. This is due to the Reducing Sugars that were produced from the hydrolysis of the Non-Reducing Sugar.
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